KH2Po4 vs. K2HPO4: Ho utloisisa liphapang tsa sehlooho Buffers

Sengoliloeng sena se hlalosa phapang lipakeng tsa KH2Po4 (potastim diyhydrogen Phoosphate) le k2po4) tharollo ea pusphate), lisosa tse peli tse tloaelehileng tsa tharollo ea pusphate. Re tla qala thepa ea bona ea lik'hemik'hale, e sebetsa joang buffers, le joang ho khetha e nepahetseng bakeng sa litlhoko tsa hau tse khethehileng. Hore na u mofuputsi ea khethiloeng kapa u qala ho qala lab, ho utloisisa phapang ena ho bohlokoa molemong oa liteko. Sena ke a E tlameha ho bala Bakeng sa mang kapa mang ea sebetsang le tharollo ea buffer ho biology, k'hemistri, kapa likarolo tse amanang.

Buffer ea phosphate ke eng? Tlhaloso

A buffer Tharollo ke sesebelisoa sa bohlokoa litekong tse ngata tsa mahlale. Mosebetsi oa eona o moholo ke ho hanela liphetoho ho PH ha e nyane asiti kapa motheo ke ekeletsoa. Sena se bohlokoa hobane maemo a mangata a lik'hemik'hale, haholo-holo a sistimi ea likokoana-hloko, o tsotella haholo ho liphetoho haholo.

Li-buffers tsa phosphate, ka ho khetheha, e sebelisoa haholo hobane ba khona buffer holim'a boleng bo bongata ba ph mme ba lumellana le litsamaiso tse ngata tsa likokoleche. Li entsoe ka mefuta e fapaneng ea Phosphate, molek'hule e nang le phosphorus le oksijene. E tloaelehileng Phosphate buffer May e na le motsoako oa KH2Po4 (Potium Dihydrogen Phosphate) K2HPO4 (Dipotasiamom Fydrogen phosphate. Karolelano e khethehileng ea likarolo tsena tse peli e khethollang ho qetela PH tsa buffer.

Phapang ke efe pakeng tsa KH2Po4 le K2HPO4?

Senotlolo Phapang lipakeng KH2Po4 mme K2HPO4 Mashano ka palo ea haedrojene (H) liathomo li e na le.

• KH2Po4 (Lebon Popastium phosphaten. E na le tse peli haedrojene liathomo. Ha e qhibiliha ka metsing, e sebetsa joaloka se fokolang asiti, ho fana ka proton (h +) ho Tharollo.

• K2HPO4 (Dipotassium Fydrogen Phosphate): Motsoako ona o boetse o tsejoa e le Dibasic Popastium phosphaten. E na le e le 'ngoe feela haedrojene athomo. Ha e qhibiliha ka metsing, e sebetsa e le motheo o fokolang, o amohela proton (H +) ho tloha ho Tharollo.

Phapang ena e nyane ka sebopeho sa lik'hemik'hale e lebisa ho liphapang tse kholo mabapi le boitšoaro ba bona ka tharollo. KH2Po4 e kenya letsoho ka har'a thepa ea acid ea buffer, ha K2HPO4 e kenya letsoho ho mantlha (kapa Alkaline) thepa.

U sebetsa joang le k2po4 e sebetsa 'moho Tharollo ea buffer?

KH2Po4 mme K2HPO4 sebetsa hammoho e le para ea acid-setsi sa acid ho theha a Phosphate buffer. Karabelo ea tekano e ka emeloa tjena:

H2po4- (aq) + h2o (l) ⇌ Hpo42- (aq) + h3o + (aq) +

• KH2Po4 e fana ka h2po4- (Dinhydrogen Phoosphate) li-ion.
• K2HPO4 e fana ka hlo42- (Fydrogen phosphate) li-ion.

Ha palo e nyane ea asiti (H +) ke ekeletsoa ho buffer, li-HPO42- Liion li arabela ka asiti, a fetolela karolo e ka letsohong le letšehali le ho fokotsa phetoho ho PH. Ha palo e nyane ea motheo (Oh-) ke ekeletsoa, li-H2po4 li arabela ka motheo, li fetohela ho lekana ka ho le letona mme hape ho fokotsa phetoho ho PH. Tsebo ena ea ho hanela liphetoho tsa PH ke eona e etsang buffer E na le thuso e joalo. Karolelano ea tla eketsa molemong oa.

U ka lokisa tharollo ea pusphate buffer ea phosphate buffer e nang le kh2po4 le k2HPO4?

Ho Itokisetse a Phosphate buffer Tharollo, O tla hloka:

1. KH2Po4 (potasiamo pibydroen phosphate)
2. K2HPO4 (Dipotasiamom Fydrogen phosphate)
3. Metsi a Lekiloeng
4. Ph Meter
5. Beakers le lisebelisoa tse hlohlelletsang

Ketsahalo ena e akaretsang (e lula e le ntho e itseng Protocol Bakeng sa ho lakatsa PH mme TLHOKOMELISO:

1. Lekola PH le khatello ea maikutlo a hau. Mohlala, u ka batla 0.1m Phosphate buffer ho PH 7.2.

2. Bala palo ea kh2po4 le k2hpo4 tse hlokahalang. U ka sebelisa Henderson-hasselbank tekano kapa inthaneteng buffer li-calculator ho fumana tse nepahetseng karolelano ea likarolo tse peli. Equation ea henderson-hassebal ke:
   PH = pka + log ([HPO42 -] / [H2PO4-]
   Moo pka e amanang le Phosphate Ion (hoo e ka bang 7.2 bakeng sa ho feto-fetoha ha phosphoric asiti.

3. Bala mese ea kh2po4 le K2Po4 ho buffer, ebe eketsa boima ba 'mele oa lerōla le tla u joetsa hore na ligrama tse kae li tla eketsa ho Tharollo.

4. Qhala Matšoele a Balitseng a KH2Po4 mme K2HPO4 Moqolotsi oa metsi a qheletsoeng a le tlase ho feta kamoo u batlang Buka. Mohlala, haeba u batla litara e le 1 ea buffer, qala ka hoo e ka bang 800 ml ea metsi.

5. Tsosa tharollo ho fihlela letsoai le qhibiliha ka botlalo.

6. Sebelisa PH Meter ho lekanya PH ea tharollo.

7. Haeba ho hlokahala, fetola pH ka ho eketsa tharollo e nyane ea khkopo4 (ho theola phallo) kapa ho holisa ngaka).

8. Hang ha Ph ph ph ph ph e tsebahalang e fihletsoe, eketsa metsi a behiloeng a ho tlisa tharollo ea molumo oa ho qetela o lakatsehang.

Ph ph bry of a phosphate buffer?

Li-buffers tsa phosphate li sebetsa hantle haholo ho PH mefuta e ka bang 6.0 ho isa ho 8.0. Ke hobane pka ea Fydrogen phosphate/ diyhydrogen Phosphate equilibrium e haufi le 7.2. The bufffer e phahame haholo ha PH e haufi le boleng ba pka. Le ha e sebetsa haholo le 7.2 e ka khona buffer Litekanyetsong tsa boleng bo fapaneng, ho kenyeletsoa hanyane Alkaline 7.4.

Leha ho le joalo, ho bohlokoa ho hlokomela hore e sebetsang buffer Libaka e ka ata haholo ho latela mamello e amohelehang ea PH fetoha ka kopo e itseng. A Phosphate buffer e kanna ea ba e fana ka tse ling buffer bokhoni ka ntle ho mofuta ona, empa ho ke ke ha sebetsa-tlase ho hanyetsana PH Liphetoho. The Phosphate buffer Range e loketse lits'ebetso tse ngata tsa tlhaho.

Ke khetha joang pakeng tsa KH2Po4 le K2HPO4 bakeng sa teko ea ka?

Khetho pakeng tsa ho sebelisa KH2Po4 kapa K2HPO4 a le mong, kapa ka kopanelo, o itšetlehile ka seo a se batlang PH tsa hau Tharollo.

• Haeba u hloka acidic Tharollo, o tla sebelisa haholo KH2Po4.
• Haeba o hloka oa mantlha kapa Alkaline Tharollo, o tla sebelisa haholo K2HPO4.
• Haeba o hloka ho se nke lehlakore kapa le haufi PH, o tla hloka ho sebelisa a Kopanya tsa bobeli KH2Po4 mme K2HPO4 ho theha a buffer. Ho hlakile karolelano ea tse peli li tla itšetleha ka tse ikhethang PH o leka ho fihlella.

Ke seoelo sa ho sebelisa e le 'ngoe feela ea metsoako ena setulong sa lipatlisiso. Hangata, o ikemiselitse ho theha a buffer Tharollo ea ho tsitsisa PH ea karabelo kapa Tharollo.

A na nka sebelisa acid ea phosphoric acid (h3po4) ho etsa buffer ea phosphate?

Ee, u ka sebelisa Phosphoric acid (H3po4) ho Itokisetse a Phosphate buffer. Leha ho le joalo, Phosphoric acid ke terompeta asiti, ho bolelang hore e na le liathomo tse tharo tse ntle tsa hydrogen. Sena se lebisa mehatong e meraro e fapaneng e fapaneng, e 'ngoe le e' ngoe e nang le boleng ba eona ba pka:

1. H3PO4 ⇌ H + + H2PO4- (PKA1 ≈ 2.15)
2. H het h + he + he he +
3. Hpo42- ⇌ H + + Po43- (pka3 ≈ 12.35)

Ho etsa buffer u sebelisa H3po4, u tla ratana eketsa setsi se matla, joalo ka Koh (potasiamo hydroxide) kapa Sodium hydroxide (Naoh), ho lula u nyenyefatsa asiti mme o iketsetse karolelano tsa Phosphate mefuta. Mohlala, ho theha a buffer ho potoloha PH 7, o ne o tla eketsa motheo o lekaneng ho fihlela mohato oa bobeli oa ho tloha, o theha motsoako oa H2Po4- LE HPO42-. The buffer sebaka sa Phosphoric acid e isa libakeng tse ngata.

U sebelisa H3po4 e ka ba ea rarahane ho feta ho sebelisa KH2Po4 mme K2HPO4 ka ho toba, joalo ka ha u hloka ho laola boholo ba motheo ekeletsoa ho fihlela ka hloko PH. Leha ho le joalo, e ka ba mokhoa o sebetsang haeba o ka ba joalo feela Phosphoric acid fumaneha, kapa o batla ho theha a Tharollo ka holimo Matla a Ionic.

Hobaneng kno2po4 ke acid le k2HPO4 ke mantlha?

Acidity ea KH2Po4 le motheo oa K2HPO4 Pheta ka kotloloho le meaho ea bona ea lik'hemik'hale le hore na e sebelisana le metsi joang.

• K.Po4 (potastim dibyhydrogen Phosphate): Neng KH2Po4 qhibiliha ka metsing, ea itšilafaletsa k + Ions le H2po4- Ions. The Dinhydrogen Phoosphate Ion (H2po4-) e ka sebetsa joalo ka ba fokolang asiti, ho fana ka proton (H +) ho metsi:
  H2po4- + H2O ⇌ Bo42- + H3O +
  Sebopeho sa h3O + (li-Hydronium tsa hydronium) e eketsa asiti tsepamisa maikutlo ho Tharollo, e etsa joalo.

• K2Po4 (Dipotassim Fydrogen Phosphate): Neng K2HPO4 e qhibiliha ka metsing, e tlosa lihekthere tsa 2K + Ions. The Fydrogen Phoshate Ion (HPO42-) e ka sebetsa e le setsi se fokolang, se amohela setlama (H +) ho tloha metsing:
  Hpo42- + h2o ⇌ H2po4- + Jo-
  Ho thehoa ha Oh-(Hydroxide its) E eketsa motheo TLHOKOMELISO ho Tharollo, e etsa hore e be motheo kapa Alkaline.

Mokhoa oa ho fetola pH ea buffer ea phosphate?

Ho fetola PH ea Phosphate buffer e tloaelehileng mokhoa ho lab. Mona ke kamoo o ka e etsang:

1. Lekanya PH ea pele: Sebelisa li-imer tse halikiloeng ho ho lekanya ka nepo PH tsa hau buffer Tharollo.
2. Etsa qeto mabapi le tataiso ea liphetoho: Fumana hore na o hloka ho eketsa kapa ho fokotsa PH.
3. Kenya tharollo e nepahetseng:
   • Ho theola PH (ho e etsa acidic): Butle butle eketsa setopo Tharollo tsa KH2Po4 kapa semolao Tharollo ea matla asiti joalo ka Hcl (hydrocloric asiti), ha a ntse a lekola PH Ka Meter ea PH.
   • Ho phahamisa pH (ho e etsa ho feta / alkaline): Butle butle eketsa setopo Tharollo tsa K2HPO4 kapa semolao Tharollo ea motheo o matla joalo ka Koh (potasiamo hydroxide) kapa Naoh (Sodium hydroxide), ha a ntse a lekola PH Ka Meter ea PH.
4. Kopanya ka botlalo: Netefatsa hore Tharollo e tsoakiloe hantle kamora ho kenyetsoa.
5. Ema ha PH e lakatsehang e fihlelloa: Tsoela pele ho eketsa phetoho Tharollo Keketseho e nyane ho fihlela PH Meter e bala ka hloko PH boleng. Hlokomela hore u se ke oa apara.

TLHOKOMELISO EA Bohlokoa: Kamehla eketsa Ho fetolela Tharollo butle butle le ka litefiso tse nyane, ha a ntse a hlohlelletsa le ho lekola PH. Sena se thibela haholo PH Liphetoho 'me li netefatsa buffer e boloka eona bufffer. O ka bua Kand's Chemical's Nummem Sodium Acetate Litokomane tsa ho kopanya mekhoa e metle ka lik'hemik'hale tse tšoanang.

Lipontšo tse ling tse tloaelehileng tsa li-buffer tsa phosphate li fe?

Li-buffers tsa phosphate li etsahetse ka mokhoa o makatsang 'me li sebelisoa likopo tse fapaneng tsa likopo, ho kenyelletsa:

• Patlisiso ea tlhaho: Ho boloka PH tsa litso tsa sele, protheine Tharollo le boithabiso ba enzyme. Tharollo ea PSS, ka mohlala, ke Phosphate saline.
• Biology biology: DNA le Rna ho tsoa, ​​elektrophoresis, le tse ling Molecular baeloji Mekhoa ea.
• Biochemistry: Ho ithuta enzyme Kinetics, protheine tlhoekiso, le lits'ebetso tse ling tsa biochemical.
• Khemistri: Joalo ka buffer maemong a lik'hemik'hale le likarolo.
• Indasteri ea meriana: Ho theha lithethefatsi le meriana.
• Indasteri ea lijo: Ho Laola PH Ts'ebetsong ea lijo le poloko.
• Litlhapi tsa metsi a Liindasteri: Lik'hemik'hale tsa Kand's li fana ka mefuta e fapaneng ea phosphates Hangata li sebelisoa kalafo ea metsi.

Setsi sa biocompitatitatian le se ka thata PH mefuta ea Phosphate buffers Ba etse sesebelisoa sa bohlokoa masimong a mangata a fapaneng. Tse ikhethang TLHOKOMELISO mme PH tsa buffer e tla khethoa ho latela litlhoko tsa kopo e itseng.

Ho rarolla mathata a phosphate buffer

Mathata a mang a tloaelehileng a thulana le ho lokisa Phosphate buffers le ho li rarolla:

• PH ha e tsitsitse:

  • Etsa bonnete ba hore PH miter ea hau e lula hantle hantle. Sebelisa Calibration e ncha li-buffers le ho latela litaelo tsa moetsi.
  • Netefatsa hore matsoai a felisitsoe ka botlalo. Hlohlelletsa Tharollo ka botlalo ho fihlela ha ho na likarolo tse tiileng.
  • Sebelisa lik'hemik'hale tse phahameng tse phahameng, tse hloekileng. Likotsi li ka ama PH mme bufffer. Kand's Girl's Glamisi inela ka bohloeki.
  • Lekola tšilafalo. Etsa bonnete ba hore khalase ea hau ea khalase ea hau le metsi li hloekile ebile li se na litšila.
  • Na u kentse likarolo tsohle? Lekola ho bona hore e nepahetse Mass bakeng sa likarolo tsohle.

• PH e phahame haholo kapa e tlase haholo:

  • Hlahloba lintlha tsa hau habeli. Etsa bonnete ba hore u sebelisitse chelete e nepahetseng ea KH2Po4 mme K2HPO4.
  • Fetola PH ka hloko Ho sebelisa tharollo ea KH2Po4 (ho theola PH) kapa K2HPO4 (ho phahamisa PH), kapa bokhabiso Hcl kapa Koh joalo ka ha ho hlalositsoe kaholimo.

• Preticication Foromo ea Buffer:

  • Sena se ka etsahala haeba ho shejoa ha buffer e phahameng haholo. Leka ho hlasimolla buffer.
  • Litlolo tse ling tsa phosphate li na le linyeoe tse fokolang. Etsa bonnete ba hore ha u fetise moeli oa alloubility oa letsoai O sebelisa.
  • Mocheso o khona ho ama eallo. Tse ling Phosphate Li-letsoai ha li qhibiliha ka mocheso o tlase.
  • TLHALOSO. Etsa bonnete ba hore oa hau lik'hemik'hale Liphetho li lokolohile ho silafatsa, le hore u sebetsa maemong a sa lokang, ntle le litloholo tsa kantle ho naha.

• Ha e khone ho fumana ph

  • Haeba u latetse a Protocol 'me ha u fihlelle ph, leka ho sheba Marang-rang. Patlisiso e ba le sechaba se tiileng sa bo-rasaense ba arolelanang liphihlelo tsa bona, 'me u ka fumana Tlhaloso. Haeba potso mabapi le puffer ea hau ea PHOSPhate e ne e se e botsoa, u ka pheta potso ea hau ho e tšoanang.

Key Picaways

• KH2Po4 (Potasiamo Dinhydrogen Phoosphate) le K2HPO4 (Dipotasiamom Fydrogen phosphate) ke likarolo tsa bohlokoa tsa Phosphate buffers.
• KH2Po4 ke acidic, ha K2HPO4 ke ea mantlha.
• The karolelano tsa KH2Po4 mme K2HPO4 e etsa qeto ea PH tsa buffer Tharollo.
• Li-buffers tsa phosphate li sebetsa ho PH mefuta e meng ea 6.0 ho isa ho 8.0.
• U ka khona Lokisetsa lithuno tsa phosphate u sebelisa KH2Po4 mme K2HPO4, kapa ka ho ba qaqisa Phosphoric acid (H3po4) ka setsi se matla.
• Hlokolosi PH Phetoho le ho rarolla mathata a bohlokoa bakeng sa katleho buffer Ho itokisetsa.
• Haeba u ntse u lokisa buffer ho H3po4 Tlas'a Koh ho fihlela Tharollo e fihlela ph e lakatsehang.
• Ho tloha KH2Po4 ho K2HPO4 o tla hloka eketsa Koh.
• Bakeng sa morao, sebelisa Hcl.

Tataiso ena e felletseng e fana ka motheo o tiileng oa kutloisiso le ho sebelisa Phosphate buffers mosebetsing oa hau. Hopola ho lula re buisana le litataiso tse khethehileng tsa protocol le litataiso tsa polokeho bakeng sa liteko tsa hau. Mahlohonolo.