Ammonium Acetate, imiti uruganda hamwe na Cas umubare 631-61-8, ihagaze nk'ibifuni mu masomo atandukanye y'inganda na laboratoire. Kuva ku nshingano zayo nka a buffer in Hplc ku gikorwa cyayo nka an Acibity Regiator mu biryo, iyi Umunyu wa amonium ni bitandukanye bidasanzwe. Muri iki kiganiro, tuzasesengura ubujyakuzimu Hygroscopic ikomeye, Gusuzuma byayo synthesis, imiti, kandi imikoreshereze yagutse ikoresha itanga ntangarugero. Waba uri chimiste, umukozi ushinzwe amasoko, cyangwa amatsiko gusa kubyerekeye chimie idasanzwe, gusobanukirwa Gukoresha Ammonium Acetate ni byiza igihe cyawe. Tuzibira cyane uko ibi reagent kuborohereza misa, SIDA muri Gukuramo ADN, kandi ikora nkibintu byingenzi muri synthesis.
NIKI GUTANGA ACONUGUM (CAS 631-61-8)?
Ammonium Acetate ni a imiti hamwe na formula nh₄ch₃co₂. Nibyiza, byerekana umunyu wa aside ikomeye (acide ya acetic) na a Intege nke (ammonia). Uku guhuza bidasanzwe bitanga ibiranga bitandukanye bihabwa agaciro cyane muri chimie. Bitandukanye n'unyunyu sulfate cyangwa chloride ishingiye ku bigize, Ammonium Acetate Dufashe ahantu hatabogamye, akenshi ikoreshwa mugihe ibidukikije bidahungabanye, abitonda bya Ionic bisabwa. The Kas 631-61-8 kuranga bivuga cyane umunyu muburyo bwiza.
Ku mubiri, Ammonium Acetate ni umweru, Crystalline ikomeye. Birazwi ko ari a Hygroscopic ikomeye, bivuze ko bikurura ubuhehere mu kirere. Niba usize ikibindi cya Ammonium Acetate Fungura, urashobora gusanga uhindagurika hamwe cyangwa uhindukirira akajagari mugihe. Kubera iyo mpamvu, kubika neza mubidukikije byumye ni ngombwa. Ni byinshi gusohora mu mazi, kurema an Ammonium acetate Nibyo byingenzi mubikorwa byinshi bishingiye kumazi muri laboratoire.
Mw'isi y'Ubucuruzi n'inganda, Ammonium Acetate igurishwa kenshi kandi ikoreshwa nkugusukuye cyane reagent. Kuko igizwe acetate na amonimium Ion, ituma byoroshye ku gushyushya, niwo mutungo utandukanya nubwinyungo buhamye nka Sodium acetate. Iyi nkunga yubushyuhe mubyukuri ni inyungu mubuhanga bwihariye bwo gusesengura, kubuza kubaka ibisigazwa mubikoresho byoroshye.
Nigute synthesis ya amonium acetate yagezweho?
Uwiteka synthesis ya Ammonium Acetate ni acide ya kera. Ni mbere na mbere Byakozwe na statuslies ya acide ya acetic hamwe ammonia. Iyi nzira irashobora gukorwa hakoreshejwe kwibanda ku buryo butandukanye, ariko uburyo busanzwe bwinganda burimo kuzura acide hamwe ammonia. Acide ni uburyo butarimo amazi, bwibanze bwa acide ya acetic. Ryari ammonia gaze irabyibushye binyuze muri yo, the reaction ya ammonia na acide ya acetic gutanga umusaruro ukomeye Ammonium Acetate.
Ubundi buryo kuri Ammonium Acetate Imyiteguro bikubiyemo reaction ya acide ya acetike hamwe na amonium carbonate. Muri iki gihe, acide ya acetic react hamwe na karubone, kurekura dioxyde de carbone namazi mugihe ushizeho acetate. Ubu buryo bukoreshwa cyane mugihe ukoresha gase ammonia ntabwo bishoboka cyangwa iyo bikomeye amonium isoko irahitamo. Ikigereranyo Cyimiti muri rusange gisa nkibi: $ 2 ch_3cooh + (nh_4) _2Co_
Birashoboka kandi kunyeganyega mukuvanga acide yinda ya acetic hamwe na ammonia muri an Igisubizo gitangaje, nubwo kubona kristu ikomeye isaba guhimba amazi, bishobora kuba amayeri kubera impengamiro yo guhagarika ammonia Amaze gushyushya. Kubwibyo, kugenzura kwitondera ubushyuhe na PH birasabwa mugihe synthesis Kugirango ibicuruzwa byanyuma ari byiza Ammonium Acetate. Ibi reaction ya ammonia na acetike ibice nibyingenzi kubyara acetate bikenewe ku masoko ku isi.
Nibihe bintu by'ingenzi bya chimique byerekana iki kigo?
Gusobanukirwa imiti ya Ammonium Acetate ni ngombwa kugirango usabe neza. Nkuko byavuzwe, bikomoka kuri a aside ikomeye kandi ifite intege nke Shingiro. Ibi bituma umukandida mwiza wo gukora a Igisubizo cya Buffer. An acetate buffer bikozwe hamwe Ammonium Acetate Imirimo neza muri acide, gufasha guhagarika PH yivanze. Kuberako cation zombi (ammonium) na anion (acetate) irashobora hydrolyze, phi yumunyamuryango Ammonium acetate ni kutabogama, mubisanzwe hafi ya PH 7.0, nubwo ibi bishobora gushishikara bitewe no kwibanda.
Imwe mu isobanura cyane imiti ni uko ari Ihindagurika. Ibi bitandukanye sodium acetate cyangwa Potasiyumu acetate, ziva ibisigi bihamye mugihe bidasobanutse. Ryari Ammonium Acetate irashyuha cyangwa ishyirwa munsi yurubuga, itandukana Ammonia na acide ya acetic (cyangwa acide ya acetike hamwe na ammonia imyuka), hasigara hafi ntakurikiranya inyuma. Uyu mutungo ni "amayeri" ayiza cyane mubuvuzi bwisesengura.
Bijyanye Kudashoboka, Ammonium Acetate ni bitandukanye bidasanzwe. Bizakora gushonga byoroshye mumazi, methanol, na ethanol. Iyi ndende Kudashoboka Emerera gukoreshwa muburyo butandukanye Ibyiciro bya mobile kuri chromatografiya. Ariko, ntabwo bihamye kuruta Ammonium sulfate; Niba uhuye nubushyuhe igihe kirekire, kitakaza Aminia kandi gihinduka acide.
Kuki Ammonium acetate unegura muri HPLC na Misa ya Spectrometry?
Mu murima wo gusesengura chimie, cyane cyane-Imikorere yamazi ya chromatografiya (Hplc) na Hplc Huza hamwe na misa ya spectrometry (LC-MS), Ammonium Acetate ni superstar. Ni henshi ikoreshwa mu gukora buffer kuri Ibyiciro bya mobile. A. buffer kubice bigendanwa ifasha kubungabunga PH yo kwibeshya nkuko iva mu nkingi, kureba ko ibice bisesenguye (gusesengura) guma muri leta ihamye. Ibi biganisha ku mpinga zikarishye hamwe namakuru yukuri.
Impamvu nyayo Ammonium Acetate kumurika muri LC-MS niyo ihindagurika ryayo. Ryari Gutegura ingero zo kwidagadura, abahanga bagomba kwirinda Umunyu udahindagurika mugutegura ingero. Buffers gakondo ya fosiporo, mugihe ikora neza, ni umunyu ukomeye uzafunga isoko yoroshye yo gufata isoko rusange. Ammonium Acetate, kuba Ihindagurika, kugabanuka kandi birazimira. Itanga imbaraga za ioonic iine hamwe nubushobozi bubi mu cyiciro cyamazi ariko bikabura muri staptor.
Umunyu wakoreshejwe Kera, nka fosifate cyangwa sulfate, ubu basimbuwe ahanini na Ammonium Acetate cyangwa Ammonium ikora Muri iyi porogaramu. Birahuye nabyo Icyiciro cya HPLC hamwe na Elsd (Guhinduranya urumuri rworoshye gutahura), ubundi buhanga busaba buffers ihindagurika. The acetate Ion yorohereza ionisation molekile zitandukanye, ziyikora bisanzwe reagent kuri Isesengura ryibice bitandukanye kuva mu biyobyabwenge kugera kuri poroteyine.
Nigute ukora Ammonium Gutegura muri laboratoire?
Mugihe benshi bagura premixed, bazi Kora igisubizo cya buffer ni ubuhanga busanzwe bwa laboratoire. Ammonium Acetate Imyiteguro Mubisanzwe muri laboratoire bikubiyemo gushonga neza amazi meza mumazi meza. Kurugero, niba wowe bakeneye gukora amonium 10m acetate igisubizo cyimigabane (kirimo kwibanda cyane), wapima umubare munini wa Hygroscopic ikomeye na gushonga nitonze.
Ariko, kubera ko hygroscopique, ipima irashobora kuba idahwitse niba yakoresheje amazi. Rimwe na rimwe, Abami bahitamo gukora buffer muri kuvanga acide ya acetic na ammonia (cyangwa ammonium hydroxide) Ibisobanuro kugeza PH yifuzwa bigerwaho. Niba wowe kubara amafaranga asabwa Kubuntu runaka, burigihe ubazwa uburemere bw'amazi niba bikomeye byarangije.
Reka tuvuge ko ukeneye amajwi yihariye. Urashobora kubara amafaranga asabwa kuri ml 70 cyangwa umubare bisabwa ku gisubizo cya 70 y'ubuntu bwihariye. Washonga Ammonium Acetate mumazi make, guhindura ph ukoresheje acide ya acetic cyangwa ammonia, hanyuma hejuru hejuru kugeza ku bwinshi. Ibi bireba ibyawe acetate buffer ni byiza. Iki gisubizo gikunze gushungura kugirango ukureho ibintu byose mbere yo gukoreshwa nka a reagent mu buryo bworoshye.
Ammonium Acetate ikoreshwa nkibiribwa?
Nibyo, bitangaje kuri bamwe, Ammonium Acetate ni ikoreshwa nk'ibiryo byongera. Munganda zibiribwa, zikora cyane cyane nka an Acibity Regiator. Iri kurutonde rwa E Umubare E264 muburayi (nubwo imiterere yemewe iratandukanye n'akarere, bityo rero reba amabwiriza yaho). Uruhare rwayo nka an ongeraho nka acide Ifasha kugenzura PH y'ibiryo bitunganijwe, kubabuza kuba acide cyane cyangwa alkaline nyinshi, bishobora kugira ingaruka kuri france no kubungabunga.
Mugihe atari uwyititous nkuko Acide cyangwa sodium acetate, Ammonium Acetate ikoreshwa muburyo bwihariye ibiryo Porogaramu aho umukozi wigenga asabwa ko yongeraho uburyohe bwumunyu (nka sodium chloride ya sodium). Mubisanzwe bifatwa nkumutekano mubwinshi ikoreshwa nk'ibiryo Ibigize. The acetate ibice bisanzwe metabolized numubiri, na amonium itunganijwe n'umwijima.
Uwiteka Gukoresha Ammonium Acetate Mu biryo byerekana umwirondoro wumutekano wikigo iyo ukemuwe neza. Bikora kimwe nuburyo vinegere (acide ya acetic) ikora, ariko hamwe numurongo wa ph ph ugaragara kuri amonium kuringaniza-ion. Niba ikoreshwa nk'ibiryo byongera cyangwa muri synthesis yinganda, chimie yibanze ya acetate Itsinda rikomeje kuba ibintu bikora bihamye.
Ni uruhe ruhare rukina muri synthesi ya kama?
Ammonium Acetate ni ugukundwa reagent ku bahanga mu ngengo. Byakoreshejwe cyane nka a Inkomoko ya Ammonia Mubyitwaramo kuko byoroshye gupima kuruta Ammonia ya Gase. Kimwe mubyiciro byayo bizwi cyane ni nka a Catalist muri KnoevenaGel Congensation. Muri Knoevenagel reaction, Ammonium Acetate ifasha koroshya guhuza aldehyde cyangwa ketone hamwe ninkomoko yimyuga. The acetate Ibikorwa nkikigo cyoroheje cyo kwanduza hydrogène ikora, mugihe amonium irashobora gukora itsinda rya karubone.
Ubundi buryo busanzwe buri muri Borch reaction. Uwiteka Borch reaction ni inzira yo kugabanya. Hano, Ammonium Acetate ikora nka Inkomoko ya Ammonia guhindura ketone cyangwa aldehyde muri amine. The Ammonia muri borch reaction Witondere na karbonyl kugirango ikore imine, hanyuma igabambuke kuri amine. Ubu ni uburyo busanzwe bwo guhuza imiti iringaniye.
Byongeye kandi, Ammonium Acetate ikoreshwa muri synthesis ya Imidanzules, Oxazoles, nibindi bice byizurukiro. Itanga azote (kuva amonium) nubushobozi buke (kuva acetate) bikenewe kubitekerezo bigoye. Kenshi na kenshi, Ammonium acetate mu butanduye Ifishi irahitamo amonium chloride cyangwa sulfate kuko acetate Byproduct ntabwo ari intangarugero cyangwa byoroshye gukuraho.
Nigute ikoreshwa mugusesengura ubutaka nubuhinzi?
Mu rwego rw'ubuhinzi, Ammonium Acetate ugira uruhare runini mu gusesengura uburumbuke bwubutaka. By'umwihariko, ikoreshwa kuri Kwemeza potasiyumu iboneka (K) mu butaka. Uburyo burimo gukoresha aho kutabogama Ammonium acetate gukuramo ibicuruzwa bya potasiyumu kuva mubutaka. The amonium ion ikora nka a Gusimbuza CATIL ya Betasiyumu ku bumba bw'ibumba.
Kuko amonium ion (nh₄⁺) na potasiyumu ion (k⁺) zifite ingano n'amafaranga, amonium irashobora kwimura inyama zatari kubutaka. Potasiyumu amaze kurekurwa mu gisubizo, abahanga murashobora kuyipima kugirango bamenye uko ifumbire ikeneye. Iyi nzira yishingikiriza kuri ubutaka aho ibihimbano Ion ikora nkumusimbura umukozi.
Ubu buryo bwo gukuramo nuburyo busanzwe mubumenyi bwubutaka. Ifasha mugugena "ubushobozi bwo kuvunahana amakuru" (CEC) yubutaka. Mugihe ibindi bisigaye bibaho, Ammonium Acetate ni Byaguhitamo Kurekura intungamubiri nziza neza kandi ikaba ikuramo ubutaka kuri PH (mubisanzwe PH 7.0). Ibi birinda iseswa rya acide ritazaboneka mubisanzwe kubimera.
Tuvuge iki ku binyabuzima bya molecular na ADN?
Mu karomba Ibinyabuzima bya MoleCumi, Ammonium Acetate nigikoresho cyizewe kuri ADN na RNA. Nyuma yubumenyi bwabanyabupfura bufunguye kugirango babone ibikoresho bya genetike, bakunze gukoresha Ammonium Acetate kwinjiza proteyine no gukuraho umwanda. Ni ingirakamaro cyane kuko bibuza ko urwogo ya DNTP (inyubako inyura ya ADN) na oligosaccharside.
Iyo bikoreshejwe Gukuramo ADN, kwibanda cyane Ammonium Acetate yongewe kuri sample. Ibi bifasha "umunyu" poroteyine, bigatuma bahanganye kugirango bashobore kumenagura mu kigo. ADN iguma mu mazi. Nyuma, Ethanol yongewe mumazi kugirango ashimishe ADN. Ammonium Acetate ikunze gushimishwa hejuru sodium acetate Muri iyi ntambwe mugihe ADN igiye gukoreshwa muburyo bwiza (nkibigosha ukoresheje inermes) kuko the amonium ion ntabwo ikunze kubuza ubwo bwoko kuruta sodium cyangwa potasium ion.
Ni kandi ikoreshwa mu gusimbuza ibigo muri protocole zimwe. Kamere yoroheje ya acetate na amonium Ions ituma yitonda mumirongo yoroheje ya ADN na RNA. Iyi porogaramu ishimangira guhuza Ammonium Acetate: Kuva tons yinganda reagent Ku mikuri mumikino yubushakashatsi bwa genetike, akamaro kayo ni nini.
Bigereranya bite nindi sanzure nka amonium.
Iyo uhisemo a buffer cyangwa reagent, Abami bami bakunze guhitamo hagati Ammonium Acetate na Ammonium ikora. Byombi ni umunyu uhindagurika wakoreshejwe muri LC-MS, ariko bafite itandukaniro. Ammonium ikora ni umunyu wa acide, ni aside ikomeye kuruta acide ya acetic. Kubwibyo, Ammonium ikora buffers nibyiza kuri ph iri hasi (hafi ya ph 3-4), mugihe Ammonium Acetate nibyiza kumurongo wo hejuru cyane (ph 4-6).
Niba ukeneye a Igisubizo cya Buffer ibyo ni acide gato, urashobora kwishingikiriza acide n'umunyu wacyo. Ariko, acide ya acetic na Ammonium Acetate akenshi bihendutse kandi bifite akamaro gahagije kubasesengura rusange-spectrum.
Ugereranije na sodium acetate, Ammonium Acetate ifite ibyiza byo guhita. Niba ukorera inzira ukeneye gukuraho umunyu nyuma, Ammonium Acetate ni we watsinze. Niba ukeneye umunyu uhamye uguma, sodium acetate ni byiza. Ukurikije Kudashoboka, byombi birashonje cyane, ariko amonium Umunyu muri rusange ni hygroscopique.
Irindi rigereranya riri kumwe Ammonium sulfate. Sulfate ni umuntu udahindagurika, umunyu ukomeye. Nibyiza kubihingwa bya proteine (umunyu) ariko biteye ubwoba kuri misa kuko ifunga imashini. Rero, Umunyu wakoreshejwe mu mateka nka sulfate basimburwa na acetate muri laborato.
Igihe cyohereza: Nov-20-2025
