Ammonium acetate hmoov, tshuaj lom neeg sib xyaw nrog tus Cas tus nab npawj 631-61-8, sawv ua pob zeb hauv ntau yam khoom siv thiab kev kuaj thiab kev kuaj. Los ntawm nws qhov tseem ceeb lub luag haujlwm ua a ntas yeej hauv HPLC rau nws txoj haujlwm ua ib Acidity Regulator Hauv cov zaub mov, qhov no ammonium ntsev yog totuslit heev. Hauv tsab xov xwm no, peb yuav tshawb txog qhov tob ntawm qhov no Hygroscopic khoom, tshuaj xyuas nws txuj ca, Tshuaj Tshuaj Muaj Zog, thiab dav array ntawm kev siv uas ua rau nws tsis tseem ceeb. Txawm hais tias koj yog tus kws ua txuj ci, tus tub ceev xwm ua tau zoo, lossis tsuas yog xav paub txog cov kev siv tshuaj lom inorganic, to taub cov Kev siv cov ammonium acetate yog nqi koj lub sijhawm. Peb yuav dhia mus tob rau hauv yuav ua li cas no reagent pab tswj Pawg Spectrometry, AIDS hauv DNA rho tawm, thiab ua hauj lwm raws li ib qho tseem ceeb hauv Organic Synthesis.
Dab tsi yog ammonium acetate (CAS 631-61-8)?
Ammonium acetate hmoov yog a Tshuaj sib txuas nrog cov mis nh₄cha₃₃₂. Qhov tseeb, nws sawv cev rau lub ntsev ntawm cov kua qaub tsis muaj zog (acetic acid) thiab a cag qaug zog (tshuaj asmaunias). Qhov no tshwj xeeb ua ke muab nws cov yam ntxwv sib txawv uas muaj txiaj ntsig zoo hauv Chemistry. Tsis zoo li cov ntsev muaj zog zoo li ncauj lossis chloride raws cov tebchaw, Ammonium acetate Nyob hauv av nruab nrab, feem ntau siv thaum tsis muaj kev cuam tshuam tsis meej, maj ionic ib puag ncig yuav tsum muaj. Tus CAS 631-61-8 Nr tus cim qhia tshwj xeeb hais txog qhov no Acetate ntsev Hauv nws daim ntawv ntshiab.
Lub cev, Ammonium acetate yog dawb, Crystalline khoom. Nws yog lub npe hu ua a Hygroscopic khoom, txhais tau tias nws tau yooj yim nqus dej noo los ntawm huab cua. Yog koj tawm ib lub thawv ntawm Ammonium acetate Qhib, koj yuav pom nws clumping ua ke lossis tig mus rau hauv ib tug slushy mess dhau sijhawm. Vim tias qhov no, kev cia khoom kom zoo nyob hauv ib puag ncig qhuav yog qhov tseem ceeb. Nws yog heev soluble hauv dej, tsim ib ammonium acetate kev daws teeb meem Qhov no yog qhov tseem ceeb rau ntau cov txheej txheem ua kua hauv chav kuaj.
Hauv ntiaj teb Kev Lag Luam thiab Kev Lag Luam, Ammonium acetate yog nquag ua lag luam thiab siv raws li lub siab-huv reagentCov. Vim tias nws yog tsim los ntawm acetate thiab ammonium ions, nws decomposes kuj yooj yim thaum cua sov yooj yim, uas yog ib qho cuab yeej uas tau muab nws cais tawm ntawm cov ntsev ruaj khov zoo li Sodium acetateCov. Qhov no thermal instability yog qhov tau txais txiaj ntsig nyob rau hauv kev ntsuas ntsuas kev ntsuas, tiv thaiv cov tsim ntawm cov seem hauv cov cuab yeej siv.
Tus synthesis cov acetate tiav?
Tus txuj ca ntawm Ammonium acetate yog ib qho classic acid-luj cov tshuaj tiv thaiv. Nws yog feem ntau tsim los ntawm qhov nruab nrab ntawm acetic acid nrog tshuaj asmauniasCov. Cov txheej txheem no tuaj yeem ua tiav siv cov kev ua kom ntau, tab sis feem ntau cov qauv kev lag luam yuav muaj glacial acetic acid nrog tshuaj asmaunias. Glacial acetic acid yog cov dej dawb, fullscricated daim ntawv ntawm acetic acidCov. Thaum tshuaj asmaunias roj yog bubbled los ntawm nws, tus Cov tshuaj tiv thaiv ntawm ammonia thiab acetic acid yields cov khoom Ammonium acetate.
Lwm txoj kev rau Ammonium acetate npaj koom nrog cov tshuaj tiv thaiv ntawm acetic acid nrog ammonium carbonateCov. Hauv cov xwm txheej no, acetic acid reacts nrog cov carbonate, tso cov pa roj carbon dioxide thiab dej thaum tsim lub acetateCov. Hom no feem ntau siv thaum tuav gaseous tshuaj asmaunias yog tsis ua tau los yog thaum ib qho khoom siv tus nees qhov chaw yog nyiam. Cov tshuaj lom neeg tshuaj lom neeg feem ntau zoo li no: $ 2 ch_3cooh + (nh_4co_3 \ greitarrow 2 nh_4ch_3co + CO_2 $.
Nws tseem muaj peev xwm ua tau zoo nkauj nws los ntawm kev sib xyaw glacial acetic acid nrog ammonia Hauv ib Cov tshuaj aqueous. tshuaj asmaunias Thaum cua sov. Yog li no, ceev faj tswj kev kub thiab kuaj ntev thaum lub sijhawm txuj ca Txhawm rau kom cov khoom kawg yog cov ntshiab Ammonium acetateCov. No cov tshuaj tiv thaiv ntawm ammonia thiab acetic Cheebtsam yog qhov tseem ceeb rau kev tsim cov acetate xav tau rau kev lag luam thoob ntiaj teb.
Dab tsi yog qhov tseem ceeb tshuaj tshuaj ntawm no compound no?
Nkag siab lub Tshuaj Tshuaj Muaj Zog ntawm Ammonium acetate yog qhov tseem ceeb rau nws cov ntawv thov tau zoo. Raws li tau hais, nws yog muab tau los ntawm a Cov kua qaub tsis muaj zog thiab tsis muaj zog puag. Qhov no ua rau nws yog ib tus neeg sib tw zoo rau kev tsim ntas yeejCov. Ib Acetate tsis ua nrog Ammonium acetate Ua haujlwm tau zoo nyob rau hauv cov acidic ntau, pab kom ruaj khov rau cov tshuaj tiv thaiv kab sib xyaw. Vim tias ob lub cation (ammonium) thiab anion (acetate) Hydrolryze, Ph ntawm ib qho ntshiab ammonium acetate kev daws teeb meem yog kwv yees nruab nrab ntawm pH 7.0, tab sis qhov no tuaj yeem hloov pauv raws li kev xav.
Ib qho ntawm feem ntau txhais Tshuaj Tshuaj Muaj Zog yog tias nws yog volatile ntawm qhov kev ntsuas qisCov. Qhov no yog txawv ntawm sodium acetate muaj lossis Poov tshuaj actate, uas tso tawm cov khoom seem thaum cov kuab tshuaj yaj. Thaum Ammonium acetate yog rhuab los yog muab tso rau hauv lub tshuab nqus tsev, nws cuam tshuam rov qab rau hauv ammonia thiab acetic acid (lossis acetic acid nrog ammonia vapor), tawm zoo tsis muaj kab tom qab. Cov cuab yeej no yog "Magic dag" uas ua rau nws nrov heev hauv kev tshuaj ntsuam chemistry.
Hais txog so, Ammonium acetate yog totuslit heev. Nws yuav yaj siv hauv dej, methanol, thiab ethanol. Qhov siab no so Tso cai rau nws siv hauv ntau yam Txawb ntu rau chromatography. Txawm li cas los xij, nws tsis tshua ruaj khov dua Ammonium sulfate; Yog tias raug cua sov rau ntev ntev dhau, nws loses ammonia thiab ua acidic.
Vim li cas Ammonium acetate tseem ceeb hauv HPLC thiab Mass Spectrometry?
Nyob rau hauv daim teb ntawm kev ntsuas analistry, tshwj xeeb yog high-Kev Ua Kua Chromatography (HPLC) thiab HPLC Coupled nrog huab hwm coj spectrometry (LC-MS), Ammonium acetate yog ib tug superstar. Nws yog dav siv los tsim ib tug tsis rau Txawb ntuCov. Ib ntas rau mob theem Pab tswj cov phua ntawm cov kuab tshuaj thaum nws txav mus los ntawm kem, kom ua kom cov tebchaw (analytes) nyob hauv lub xeev ionization. Qhov no ua rau muaj kev sib tw siab thiab muaj cov ntaub ntawv muaj tseeb dua.
Qhov Tiag Ammonium acetate ci rau hauv lc-ms yog nws volatility. Thaum Npaj cov qauv rau cov khoom loj, Cov kws tshawb fawb yuav tsum zam Cov ntsev tsis muaj volatile hauv kev npaj cov qauvCov. Tsoos phosphate buffers, thaum ua tau zoo, yog ntsev ua uas yuav txhaws cov kev huaj vam ntau ntawm cov huab hwm coj hluav taws xob. Ammonium acetate hmoov, ua volatile ntawm qhov kev ntsuas qis, sublimates thiab ploj mus. Nws muab cov khoom siv tsim nyog Ionicfer thiab fuffering muaj peev xwm hauv theem ua kua tab sis ploj hauv theem ntawm cov ntes.
Cov ntsev uas tau siv yav dhau los, zoo li phosphates los yog sulfates, tam sim no hloov tau ntau ntau Ammonium acetate lossis Ammonium tsim Hauv cov ntawv thov no. Nws tseem tau tshaj theem rau HPLC nrog ELSD (Evaporative lub teeb scattering ntes), lwm cov txheej txheem uas yuav tsum muaj volatile buffers. Tus acetate ion pab txhawb kev ionization ntawm ntau yam lwg me me, ua rau nws tus qauv reagent Rau Kev tsom xam ntawm ntau yam tebchaw Xws li cov tshuaj mus rau cov protein.
Koj yuav ua li cas ua ammonium acetate npaj hauv lab?
Thaum muaj ntau tus yuav nws premixed, paub yuav ua li cas Tsim kev daws teeb meem yog ib qho txuj ci tseem ceeb. Ammonium acetate npaj Hauv chav kuaj feem ntau yuav cuam tshuam nrog discrololving cov khoom siv crystalline hauv cov dej siab. Piv txwv li, yog tias koj yuav tsum tau ua 10m ammonium acetate Tshuag tshuaj (uas yog heev concentrated), koj yuav hnyav tawm qhov tseem ceeb ntawm Hygroscopic khoom thiab yaj Nws ua tib zoo.
Txawm li cas los xij, vim hais tias nws yog hygroscopic, uas nws tuaj yeem ua tsis tau yog tias nws tau nqus dej. Qee zaum, chemists nyiam ua kom cov ntas yeej Hauv situ los ntawm kev sib xyaw acetic acid thiab tshuaj asmaunias (lossis ammonium hydroxideCov kev daws teeb meem kom txog thaum lub pH uas xav tau. Yog koj Xam cov nyiaj uas yuav tsum tau ua Rau kev molarity tshwj xeeb, ib txwm suav nyiaj rau cov dej nyhav yog tias cov khoom tau txhaws.
Cia peb hais tias koj xav tau ib qho ntim khoom tshwj xeeb. Koj yuav xam cov Qhov nyiaj yuav tsum tau rau 70 ml los yog tus nqi Yuav tsum muaj 70 ml kev daws teeb meem ntawm kev ua kom pom tseeb. Koj yuav yaj cov Ammonium acetate nyob rau hauv me ntsis tsawg dua dej, kho lub pH siv acetic acid lossis tshuaj asmaunias, thiab tom qab ntawd sab saum toj mus txog rau qhov kawg ntim. Qhov no ua rau koj Acetate tsis yog meej. Txoj kev daws teeb meem no feem ntau tau lim kom tshem tawm cov partpulates ua ntej siv raws li a reagent nyob rau hauv rhiab seev.
Puas yog ammonium acetate siv los ua khoom noj ntxiv?
Yog, ceeb rau qee tus, Ammonium acetate yog siv los ua khoom noj ntxivCov. Hauv kev lag luam khoom noj khoom haus, nws pabcuam feem ntau yog ib qho Acidity RegulatorCov. Nws muaj npe nyob rau hauv tus lej e264 nyob hauv Tebchaws Europe (tab sis kev pom zoo cov txheej txheem sib txawv los ntawm cheeb tsam, yog li ib txwm txheeb xyuas cov cai hauv zos). Nws lub luag haujlwm ua ib Additive li cov kev tswj hwm acidity Pab tswj cov pheebsuab cov zaub mov, tiv thaiv lawv kom tsis txhob ua dhau los acidic lossis ib yam nkaus li ntawd, uas tuaj yeem cuam tshuam cov tsw thiab txuag.
Thaum tsis yog Ubiquitous li citric acid lossis sodium acetate muaj, Ammonium acetate yog siv nyob rau hauv tshwj xeeb mov Cov ntawv thov uas tus neeg sawv cev buffering yog qhov yuav tsum tau uas tsis ntxiv ib qho qab ntsev (zoo li cov tshuaj sib yeem). Nws yog feem ntau suav tias muaj kev nyab xeeb hauv cov khoom siv los ua khoom noj Muaj. Tus acetate tivthaiv yog ib txwm metabolized los ntawm lub cev, thiab tus tus nees yog ua tiav daim siab.
Tus Kev siv cov ammonium acetate Hauv cov zaub mov ua kom pom qhov sib xyaw ua ke ntawm cov ntaub ntawv kev nyab xeeb thaum daws tau kom raug. Nws ua zoo ib yam li cas kua iab (acetic acid) ua haujlwm, tab sis nrog ib qho ntau nruab nrab pH profile vim yog tus tus nees Txee-ion. Txawm yog siv los ua khoom noj ntxiv lossis hauv kev lag luam hluavtaws, cov khoom siv anecisation ntawm lub acetate Pab pawg tseem tshuav cov xwm txheej sib xws.
Nws ua haujlwm dab tsi hauv kev ua si hauv nruab nrog cev?
Ammonium acetate hmoov yog kev nyiam reagent rau cov organic. Nws yog dav siv raws li a Tau qhov twg los ntawm ammonia Hauv kev tawm tsam vim tias nws yooj yim dua los lis dua li gamonia. Ib qho ntawm nws cov ntawv thov tshaj plaws yog ib qho Catalyst nyob rau hauv lub knooevenagel condensationCov. Nyob rau hauv Txhos caug Cov tshuaj tiv thaiv, Ammonium acetate Pab txhawb txoj kev sib raug zoo ntawm aldehyde lossis ketone nrog cov khoom siv hydrogen compound. Tus acetate ua yeeb yam uas yog ib lub hauv paus me me rau kev ua haujlwm hydroronen, thaum lub tus nees tuaj yeem qhib pawg carbonyL.
Lwm daim ntawv thov tseem ceeb yog nyob hauv Borch tshuaj tiv thaiv. Tus Borch cov tshuaj tiv thaiv yog txheej txheem daws cov txheej txheem daws teeb meem. Ntawm no, Ammonium acetate ua hauj lwm Tau qhov twg los ntawm ammonia Txhawm rau hloov lub Ketone lossis Aldehyde rau hauv amine. Tus ammonia hauv cov tshuaj lom neeg reacts nrog lub carbonyl los tsim lub imine, uas yog tom qab ntawd txo rau lub amine. Nov yog tus qauv txheem rau kev ua suab nrov kws tshuaj hauv kev siv hluav taws xob.
Tsis tas li ntawd, Ammonium acetate yog siv nyob rau hauv synthesis ntawm imidazoles, oxazoles, thiab lwm yam heterocyclic teb. Nws muab ob lub nitrogen (los ntawm tus nees) thiab muaj peev xwm ua tsis tau (los ntawm acetate) Xav tau rau cov nyom no lub nplhaib-sib piv. Nyob rau hauv ntau kis, Ammonium acetate hauv cov ntshiab Daim ntawv yog nyiam dua ammonium chloride lossis ncauj vim hais tias tus acetate Broproduct tsis muaj kev cuam tshuam tsawg dua los yog yooj yim dua.
Yuav ua li cas yog nws siv hauv av tsom xam thiab ua liaj ua teb?
Hauv kev ua liaj ua teb ua liaj ua teb, Ammonium acetate Ua si lub luag haujlwm tseem ceeb hauv kev txiav cov av fertility. Tshwj xeeb, nws yog siv rau cov Kev txiav txim siab ntawm muaj poov tshuaj (K) hauv av. Tus txheej txheem cuam tshuam nrog kev siv nruab nrab ammonium acetate kev daws teeb meem Txhawm rau rho tawm cov poov tshuaj ions los ntawm cov av kuaj. Tus tus nees ion ua ib Hloov Cation rau Potassium Ntawm cov av cov av nplaum hais.
Vim hais tias tus tus nees ion (NH₄⁺) thiab cov poov tshuaj ion (K⁺) muaj qhov ntau thiab tsawg thiab raug nqi, tus tus nees tuaj yeem tshem tawm cov tshuaj potassium rau hauv av. Thaum lub poov tshuaj tawm mus rau hauv kev daws teeb meem, cov kws tshawb fawb tuaj yeem ntsuas nws los txiav txim siab ntau npaum li cas cov neeg xav tau. Cov txheej txheem no tso siab rau lub Cov av nyob rau Ammonium ion ua raws li hloov tus neeg sawv cev.
Txoj kev rho tawm no yog tus qauv hauv kev tshawb fawb av. Nws pab hauv kev txiav txim siab lub "cation pauv peev" (CEC) ntawm cov av. Hos lwm cov extractants nyob muaj lawm, Ammonium acetate Yog qhov zoo dua vim tias nws simulates tso tawm cov khoom noj muaj txiaj ntsig ncaj thiab buffers cov av extract mus rau qhov nruab nrab pH (feem ntau PH 7.0). Qhov no tiv thaiv cov kua qaub yaj xwm ntawm cov zaub mov uas yuav tsis muaj rau cov nroj tsuag.
Yuav ua li cas txog molecular biology thiab DNA extraction?
Nyob rau hauv lub realm ntawm Molecular Biology, Ammonium acetate yog ib tug ntseeg siab rau DNA thiab RNA limCov. Tom qab cov kws tshawb fawb tsoo cov hlwb kom nkag tau cov khoom siv caj ces, lawv feem ntau siv Ammonium acetate Yuav kom precipitate proteins thiab tshem tawm cov paug tsis huv. Nws yog muaj txiaj ntsig tshwj xeeb vim tias nws inhibits lub colrecipitation ntawm DNTPS (cov tuam tsev DNA) thiab qee cov oligosaccharides.
Thaum siv rau DNA rho tawm, lub siab siab ntawm Ammonium acetate yog ntxiv rau tus qauv. Qhov no pab kom "ntsev tawm" cov protein, ua rau lawv insoluble kom lawv tuaj yeem spun hauv ib qho kev ntseeg. DNA tseem nyob hauv cov kua. Tom qab ntawd, ethanol ntxiv rau cov kua rau nag ceev cov DNA. Ammonium acetate hmoov feem ntau nyiam dua sodium acetate muaj Nyob rau hauv cov kauj ruam no thaum DNA yuav siv rau enzymatic cov tshuaj tiv thaiv (zoo li kev zom los ntawm kev txwv cov enzymes) vim tias cov tus nees Ion tsis yog tsawg yuav txwv tsis pub inhibit cov enzymes tshaj sodium lossis potassium ions.
Nws tseem siv los hloov cov cell buffers nyob rau hauv qee cov kev cai. Tus mob me ntawm cov acetate thiab tus nees ions ua rau nws maj mam rau ntawm cov strand kiag ntawm DNA thiab RNA. Daim ntawv thov no underscores qhov versatility ntawm Ammonium acetate: los ntawm tons ntawm kev lag luam reagent rau cov microliters hauv kev tshawb fawb caj ces, nws cov nqi hluav taws xob yog loj heev.
Nws sib piv rau lwm cov sterts zoo li ammonium tsim?
Thaum xaiv ib ntas yeej lossis reagent, Chemists feem ntau xaiv ntawm Ammonium acetate thiab Ammonium tsimCov. Ob qho tib si yog cov salts salts siv hauv LC-MS, tab sis lawv muaj qhov sib txawv. Ammonium tsim yog ntsev ntawm ua yeeb yaj duab acid, uas yog lub zog acid tshaj acetic acidCov. Thiaj li, Ammonium tsim Buffers zoo dua rau qis dua hom (nyob ib ncig ntawm PH 3-4), whereas Ammonium acetate yog zoo dua rau ib qho ntau dua (Ph 4-6).
Yog tias koj xav tau a ntas yeej uas yog me ntsis acidic, koj yuav yaim ntawm ua yeeb yaj duab acid thiab nws cov ntsev. Txawm li cas los xij, acetic acid thiab Ammonium acetate Feem ntau pheej yig dua thiab txaus tsim nyog rau dav dav dav-spectrum analyzes.
Piv rau sodium acetate muaj, Ammonium acetate muaj qhov zoo dua ntawm volatility. Yog tias koj tab tom khiav tus txheej txheem uas koj yuav tsum tau tshem ntawm cov ntsev tom qab, Ammonium acetate yog tus yeej. Yog tias koj xav tau cov ntsev ruaj khov uas nyob qis qis, sodium acetate muaj yog zoo dua. Hais txog so, ob leeg yog heev soluble, tab sis tus tus nees Ntsev feem ntau ntau hygroscopic.
Lwm qhov sib piv nrog Ammonium sulfate. Ncauj yog tsis yog-volatile, muaj zog ntsev. Nws yog qhov zoo rau cov protein nag lossis daus (salting tawm) tab sis txaus ntshai rau huab hwm coj ntau vim tias nws txhaws lub tshuab. Yog li ntawd, Cov ntsev uas tau siv Keeb kwm zoo li ncauj yog raug hloov los ntawm acetate hauv kev ntsuas kev txheeb xyuas tam sim no.
Lub Sijhawm Post: Nov-20-2025
