Kh2po4 vs k2hpo4:ʻO ka hoʻomaopopoʻana i nāʻokoʻa kī i nā mea phosphate

Ua wehewehe kēiaʻatikala i nāʻokoʻa ma waena o KH2PO4 E hana mākou i loko o kā lākou mau waiwai e pono ai, pehea lākou e hana ai i nā pahu, a pehea e koho ai i kahi kūpono no kāu mau pono. Inā paha he mea noiʻi a hoʻomaka paha i ka lab, ke hoʻomaopopo nei i kēia mauʻokoʻa he mea nui loa no nā hopena hoʻokolohua kūpono. He Pono-heluhelu No nā mea hana e hana ana me Buffer Solutions i loko o Biology, Chemistry, a iʻole nā ​​hale e pili ana.

He aha kahi bhosphate? He wehewehe

A Bufer ʻO ka hopena he mea maikaʻi loa i nā hoʻokolohua koʻikoʻi he nui. ʻO kāna hana nui e pale ai i nā loli i loko ph I ka wā liʻiliʻi o nā ʻakika a iʻole kumu hoʻohuiʻia. ^. He mea nui kēia no ka mea he nui nā hopena cemical,ʻoi aku ka nui o nā'ōnaehana biological, he naʻau nui loa i nā loli ph.

Nā pōkā phosphate, ma keʻano he nui, hoʻohana nuiʻia no ka mea hiki iā lākou Bufer ma luna o kahiʻano o nā kumukūʻai ph a kūpono i nā'ōnaehana nui. Ua hanaʻia lākou me ka hoʻohanaʻana i nāʻano likeʻole o PALATEPATE, kahi molena e loaʻa ai nā phosphorus a me ka oxygen. He maʻamau Phosphate buffer paha pili i loko kahi hui o Kh2po4 (Pocassium dihydrogen phosphate) a K2hpo4 (dipotassum Heritage photo). ʻO ka pae kiko'ī o kēia mau'āpanaʻelua e hoʻoholo i ka hope loa ph o ka Bufer.

He aha kaʻokoʻa ma waena o kh2po4 a me k2hpo4?

Ke kī ʻokoʻa mawaena Kh2po4 a K2hpo4 moe ma ka helu o hydrogen (H) atom a lākou pili i loko.

• Kh2po4 (Pocassium Diydrogegen Phosphate): Uaʻikeʻia kēia pūpū he monobasic paspassate phosphate. ^. He elua hydrogen nā atom. I ka wā i hoʻokaʻawaleʻia ai i ka wai, hana ia he nāwaliwali ʻakika, hāʻawi i kahi proton (h +) i ka hopena.

• K2hpo4 (Dipotassium hydrogen phosphate):ʻikeʻia kēiaʻano lāʻau me ka dicastic paspassate phosphate. ^. Hookahi wale no hydrogen ATOM. I ka wā i hoʻokaʻawaleʻia ai i ka wai, hana ia he mea palupalu, eʻae ana i kahi proton (h +) mai ka hopena.

ʻO kēiaʻano liʻiliʻi e like me keʻano o keʻano ke alakaʻi i nāʻokoʻa nui i nāʻokoʻa nui i kā lākouʻano i ka hopena. Kh2po4 Hāʻawi i nā waiwai acidic o ka Bufer, tiha K2hpo4 Hāʻawi i ka kumu (a iʻole alkaliline) nā waiwai.

Pehea e hana pū aiʻo Kh2po4 a me K2hpo4 i kahi pahuhopu buffer?

Kh2po4 a K2hpo4 e hana pū me kahi acid-bour part-bree i hana ai i kahi Phosphate buffer. ^. Hiki ke hōʻikeʻia ka hopena o keʻano likeʻole penei:

H2PO4- (Aq) + LORO (L) ⇌ HPO42- (AQ) + AQ)

• Kh2po4 hoʻolako i ka H2po4- (ailohikuike) mau ions.
• K2hpo4 hoʻolako i ka HPO42- (Heritage photo) mau ions.

I ka manawa liʻiliʻi o ʻakika (H +) hoʻohuiʻia i na Bufer, ua hana hou ka HPO42 ʻakika, ke hoʻololi nei i ka kūlike i ka hema a me ka hōʻemiʻana i ka hoʻololi i loko ph. ^. I ka nui o kahi waihona liʻiliʻi (Oh-) hoʻohuiʻia,ʻo ka H2PO4- IS 'RETleloʻia me ke kumu, e hoʻololi ana i ka equilibrium i ka'ākau a me ka liʻiliʻi e ho'ēmi ana i ka loli i loko ph. ^. ʻO kēia hiki ke pale aku i nā loli phi Bufer No laila e pono ai. Ka pae o ka ratio Hoʻohui i ka hopena.

Pehea e hoʻomākaukau ai i kahi phosphate buffer buffer buffer me kh2po4 a me k2hpo4?

I hoʻomākaukau a Phosphate buffer ʻO ka hoʻonā, ponoʻoe:

1. Kh2po4 (ʻO Potrossaum Dihydrogen Phosphate)
2. K2hpo4 (dipotassum Heritage photo)
3. Ka wai wai
4. He mika phi
5. Nā mea mālama a me nā mea hana

Eia kekahi kaʻina hana maʻamau (e kamaʻilio mau i kahi kiko'ī protocol No kāu makemake ph a kūlike):

1. E hoʻoholo i ka pH i makemakeʻia a me ka noʻonoʻo o kāu buffer. No ka laʻana, makemake pahaʻoe i 0.1m Phosphate buffer I PH 7.2.

2. E helu i ka nui o kh2po4 a me k2hpo4 pono. Hiki iāʻoe ke hoʻohana i ka henderson-hesselbatch kaulike Opō paha pūnaewele Bufer calculators e hoʻoholo i ka pololei ʻO ka'āpana o nā'āpanaʻelua. ʻO ka hoʻohālikelikeʻo Hendersson-Hasselbatch:
   PH = PKA (LOG ([HPO42 -] / [H2po4-]
   Kahi e pili ana ka pka i ka PALATEPATE ION (Ma kahi kokoke 7.2 no ka hewaʻelua o ka phospridio ʻakika).

3. E helu i nā mole o kh2po4 a me k2hpo4 i loko o ka buffer, a laila Hoʻohui ke kaumaha o ka molar molar a e haʻi aku iāʻoe i ka nui o nā grama e hoʻohui i ka Kōkua.

4. Distsolve nā helu helu helu Kh2po4 a K2hpo4 I ka nui o ka wai i laweʻia ma lalo o kāu makemake hope Volume. ^. No kahi laʻana, inā makemakeʻoe 1 lita o Bufer, e hoʻomaka me ka 800 ml wai.

5. E hoʻomoʻi i ka hoʻonā a hiki i nā paʻakai loa i hoʻopauʻia.

6. E hoʻohana i kahi mika pr e ana i ke ana i ka pH o ka hopena.

7. Inā pono, e hoʻoponopono i ka pH ma ka hoʻohuiʻana i nā hopena liʻiliʻi o ka hopena o kahi kh2po4 (e hoʻohaʻahaʻa i ka ph).

8. Ke loaʻa nei ka mea i makemakeʻia, e hoʻohui i ka wai i laweʻia e lawe i ka hoʻonā i ka nui o ka nui i makemakeʻia.

He aha ka ph o ka ph o ka bhosphate phosphate?

Nā pōkā phosphate he mea maikaʻi loa i ka ph ʻano o ka aneane 6.0 a 8.0. ʻO kēia no ka meaʻo ka PKA o ka Heritage photo/ dihydrogen PALATEPATE ʻO ka equilibrium ma kahi o 7.2. 'Ōlelo ka mea hiki He kiʻekiʻe loa ke kiʻekiʻe ph kokoke i ka waiwai pka. ʻOiai he mea maikaʻi loa ia ma kahi kokoke 7.2 hiki Bufer I ka nui o nā waiwai, me ka liʻiliʻi alkaliline 7.4.

Eia nō naʻe, he mea nui e hoʻomaopopo i ka maikaʻi Bufer Hiki ke hoʻonui ikiʻia nā'āina ma muli o keʻano o kaʻaeʻana no ph hoʻololi i kahi noi kiko'ī. A Phosphate buffer e hoʻolako paha i kekahi pihu hiki i waho ma waho o kēiaʻano, akā e emi iho ia i ka nānāʻana ph loli. 'Ōlelo Phosphate buffer He kūpono kūpono no nā noi he nui.

Pehea e koho ai au i waena o kh2po4 a me k2hpo4 no kaʻu hoʻokolohua?

ʻO ke koho ma waena o ka hoʻohanaʻana Kh2po4 Oole K2hpo4 wale nō, a iʻole i hui pūʻia, e hilinaʻi nuiʻia ma ka makemake ph a no kau hopena.

• Inā makemakeʻoe i kahi ACIDIC hopena, e hoʻohana muaʻoe Kh2po4.
• Inā makemakeʻoe i kahi kumu a iʻole alkaliline hopena, e hoʻohana muaʻoe K2hpo4.
• Inā makemakeʻoe i kahiʻano kūponoʻole a kokoke paha ph, ponoʻoe e hoʻohana i kahi hoʻohui o elua Kh2po4 a K2hpo4 e hana ai Bufer. ^. Ka pololei ʻO ka'āpana o nā meaʻelua e hilinaʻi i ka kiko'ī ph keʻimi neiʻoe e hoʻokō.

He mea nui ia e hoʻohana wale ai i kekahi o kēia mau mea hoʻohui i kahi hoʻonohonoho noiʻi. ʻO ka pinepine pinepine, e kuhikuhi anaʻoe e hana i kahi Bufer hoʻonā e hoʻokō i ka ph o kahi hopena a iʻole hopena.

Hiki iaʻu ke hoʻohana i ka phospopric acid (h3po4) e hana i kahi bhosphate phosphate?

ʻAe, hiki iāʻoe ke hoʻohana phosphorcus acid (H3po4) i hoʻomākaukau a Phosphate buffer. ^. Eia nō naʻe, phosphorcus acid he triprotic ʻakika, keʻano heʻekolu o nā atom o nā hydrogenʻekolu. E alakaʻi ana kēia i nā hana likeʻoleʻelua, kēlā me kēia me kāna waiwai PKA pono'ī:

1. H3po4 ⇌ HP + + H2KOA4- (PKA1 ≈ 2.15)
2. H2PO4- ⇌ h + + HPO42- (PKA2 ≈ 7.20)
3. HPO42- ⇌ h + + P433- (PKA3 ≈ 12.35)

E hana ai Bufer me ka hoʻohanaʻana H3po4, e maʻamauʻoe Hoʻohui he kumu ikaika, e like me Koh (potassium hydroxide) a iʻole soitium hydroxide (Naoh), e hoʻokaʻawale i ka ʻakika a hana i ka mea i makemakeʻia ʻO ka'āpana no PALATEPATE ʻanoʻano. ʻO ka laʻana, e hana i kahi Bufer a puni ph 7, makemake pahaʻoe Hoʻohui ka nui o ka waihona e hiki ai i ka papa hana o ka lua, e hana ana i kahi hui o H2PO4- a me HPO42-. 'Ōlelo Bufer Kauka no phosphorcus acid e hoʻolōʻihi i nā'āpana he nui.

Me ka hoʻohanaʻana H3po4 hiki ke paʻakikī ma mua o ka hoʻohanaʻana Kh2po4 a K2hpo4 pololei, e like me ka pono e mālama pono i ka nui o ke kumu hoʻohuiʻia e hōʻea i ka makemake ph. ^. Eia naʻe, hiki i kahi mea kūpono ke hoʻokokoke aku inā loaʻa wale nōʻoe phosphorcus acid Loaʻa, a makemake paha e hana i kahi hopena me ke kiʻekiʻe Manao Oini.

No ke aha kh2po4 he acid a me k2hpo4ʻo ia ka kumu?

Ka acidity o Kh2po4 a me ke kumu nui o K2hpo4 E pili pono i kā lākou hana ponoʻana a pehea e kamaʻilio ai lākou me ka wai.

• Kh2po4 (potassium dihydrogen phosphate): I ka wa Kh2po4 nā'ōpala i loko o ka wai, wehe ia i loko o k + o'a me ka h2po4- h2. 'Ōlelo ailohikuike AlohaWa (H2po4-) hiki ke hana ma kahi nāwaliwali ʻakika, hāʻawi i kahi proton (h +) i ka wai:
  H2POW4- + H2O ⇌ Hpo42- + H2O +
  ʻO ka hoʻokumuʻana o H3o + (hydroum inio) hoʻonui i ka ʻakika ka nānāʻana i ka hopena, e hana ana i Acidic.

• K2hpo4 (dipotasium hydrogen phosphate): I ka wa K2hpo4 nā'ūlū i ka wai, nā dissociates i loko o 2k + a me nā ku42- mau. 'Ōlelo Holegegen Phosphate ion (HPO42-) hiki i ka hana he palupalu, eʻae ana i kahi pron (h +) mai ka wai:
  Hpo42- + + H2O ⇌ H2POO15- + Oh-
  ʻO ka hoʻokumuʻana o Oh- ​​(hydroxide Ition) hoʻonui i ka waihona kūlike i ka hopena, e hana ana i ke kumu a alkaliline.

Pehea e hoʻoponopono ai i ka pH o ka phosphate busperhate?

Hoʻoponopono i ka ph a a Phosphate buffer he mea maʻamau Kahi i ka Lab. ^. Eia pehea e hana ai:

1. E ana i ka PH: E hoʻohana i kahi leka uila calibrated e hoʻopaʻa pono i ka ph a no kau Bufer Kōkua.
2. E hoʻoholo i ke kuhikuhi o ka hoʻoponopono: Hoʻoholo inā makemakeʻoe e hoʻonui a hōʻemi paha i ka ph.
3. Hoʻohui i ka hopena kūpono:
   • E hoʻohaʻahaʻa i ka pH (e hana hou iā ACIDIC): Lohi Hoʻohui he dilite hopena no Kh2po4 a iʻole he dilite hopena o kahi ikaika ʻakika Uā HCOL (hydrochloric ʻakika),ʻoiai ke nānā mau nei i ka ph me ka mika pr.
   • E hāpai i ka pH Lohi Hoʻohui he dilite hopena no K2hpo4 a iʻole he dilite hopena o kahi kumu ikaika e like ai Koh (potassium hydroxide) a iʻole naohh (soitium hydroxide),ʻoiai ke nānā mau nei i ka ph me ka mika pr.
4. Hui pono: E hōʻoia i ka hopena ua hui pūʻia ma hope o kēlā me kēia hoʻohui.
5. Kahi i ka wā i loaʻa ai ka ph i makemakeʻia: E hoʻomau i ka hoʻohuiʻana i ka hoʻoponoponoʻana hopena I nā hoʻohui liʻiliʻi a hiki i ka leka uilaʻo PHI ph waiwai. E makaʻala eʻaʻole i overshoot.

KA MANA: Mau loa Hoʻohui ka hoʻoponoponoʻana hopena lohi a ma nā mea liʻiliʻi,ʻoiai ke hoʻomau mau nei a nānā i ka ph. ^. Mālama kēia ph nā loli a e hōʻoia i ka Bufer mālama i kāna ka mea hiki. ^. Hiki iāʻoe ke kuhikuhi ʻO Kand's Smical Sodium Actium Actium nā palapala no ka hoʻohuiʻana i nā hana maikaʻi loa me nā mea likeʻole.

He aha nā mea maʻamau o nā noi maʻamau o nā belers phsphate?

Nā pōkā phosphate heʻano maʻamau a hoʻohanaʻia i loko o kahi mau haʻawina ākea, e komo pū ana:

• Noi: Ka mālamaʻana i ka ph o nā moʻomeheu lua, huahua nā hopena, a me nā mea eʻoluʻolu. Nā hopena PBS, PALATEPATE ʻO ka paʻakai paʻakai.
• MOLECular Biology: Dna a Rna excort, electrophoresis, a me nā mea'ē aʻe molenuula loea Hana Hoʻolālā.
• Biochemistry: E aʻo ana i nā kinitics enzyme kinetics, huahua hoʻomaʻemaʻe, a me nā kaʻina hana bichemilical'ē aʻe.
• Nā Chemistry: Me he Bufer I nā hopena kaulike a me nā hōʻailona.
• ʻOihana lāʻau lapaʻau: Hanaʻana i nā lāʻau a me nā lāʻau lapaʻau.
• ʻOihana poʻeʻai. Kālai ph i ka hoʻomaʻamaʻaʻana a me ka mālamaʻana.
• ʻO ka wai hana hana: Hāʻawiʻo Kands i nā mea hoʻohālikelike i nāʻano phosoptates i hoʻohana pinepineʻia ma ka mālama wai.

Ka biocomopitibility a turomable ph Ka Haʻanuhu o Nā pōkā phosphate e hana iā lākou i kahi mea waiwai nui i nā mahina he nui. Nā kiko'ī kūlike a ph o ka Bufer e kohoʻia e pili ana i nā koi o ka noi kiko'ī.

Hoʻopilikia i ka hanaʻanaʻo Phosphate

Eia kekahi mau pilikia maʻamau i loaʻa i ka wā e hoʻomākaukau ai Nā pōkā phosphate a pehea e hoʻonā iā lākou:

• ʻAʻole paʻaʻo PH:

  • Eʻike pono i ka calibrated ponoʻo PH Meter. E hoʻohana i ka calibration hou pipili a hahai i nā'ōkuhi o nā mea hana.
  • E hōʻoiaʻiʻo i nā paʻakai loa. Hoala ae la hopena E kū mālie a hikiʻole i nā'āpana paʻaʻole.
  • E hoʻohana i ka maikaʻi kiʻekiʻe, nā mea kanu maʻemaʻe. Hiki i nā mea haumia ke hoʻopilikia i ka ph a ka mea hiki. ʻO ka lāʻau lapaʻau o Kand iā ia ma luna o ka maʻemaʻe.
  • E nānā no ka hoʻohālikelikeʻana. E hōʻoia pono i ka hoʻomaʻemaʻeʻana o kāu kīʻaha a me nā wai a me ka manuahiʻole.
  • Ua hoʻohuiʻoe i nā mea āpau? E nānā eʻike i kēlā kūpono Mamano no nā māhele āpau.

• ph he kiʻekiʻe loa a haʻahaʻa loa paha:

  • E nānā pālua i kāu helu helu. E nānā pono i ka hoʻohanaʻana i nā helu kūpono Kh2po4 a K2hpo4.
  • Hoʻoponopono i ka PH PH me ka hoʻohanaʻana i nā hopena o ka Kh2po4 (e hoʻohaʻahaʻa ph) a iʻole K2hpo4 (e hāpai ph), a iʻole e hoʻolaʻa HCOL Oole Koh E like me ka mea i hōʻikeʻia ma luna nei.

• ʻO nā puka kūpono i loko o ka buffer:

  • Hiki i kēia ke tupu inā he kiʻekiʻe loa o ka buffer. E ho'āʻo e lawe i ka Bufer.
  • ʻO kekahi mau salts phosphate ua palena i ka palena palena. E hōʻoia eʻaʻoleʻoe e hoʻonui i ka palena palena o ka aloha ke hoʻohana neiʻoe.
  • Hiki i ke aniani ke pili i ka pono. Kekahi PALATEPATE ʻoi aku ka liʻiliʻi o nā paʻakai ma nā haʻahaʻa haʻahaʻa.
  • Paio. ^. E hōʻoia i kāu Kekau ʻAʻohe o nā hoʻoponopono mai ka paleʻana, a ke hana neiʻoe i nā kūlana sterile, free mai nā mea haumia.

• ʻAʻole hiki ke loaʻa i kaʻu ph i makemakeʻia

  • Inā ua hahaiʻoe i kahi protocol aʻaʻoleʻoe e hoʻokō i ke kiʻiʻo PH, e ho'āʻo e nānā i ka Pūnaewele. Nānāhana palapala aku Loaʻa i kahi kaiāulu o nā meaʻepekema e kaʻana i ko lākou mauʻike, a loaʻa paha iāʻoe kahi wehewehe. ^. Ināʻaʻole i nīnauʻia ka nīnau e pili ana i kāu helu phosphate kūikawā nīnauʻia, paha oe pili ʻO kāu nīnau i kahi mea like.

Nā Kūleʻa Kil

• Kh2po4 (potasuma ailohikuike) a K2hpo4 (dipotassum Heritage photo) He mau mea nui o Nā pōkā phosphate.
• Kh2po4 he acidic, oiai K2hpo4 he kumu.
• 'Ōlelo ʻO ka'āpana no Kh2po4 a K2hpo4 e hoʻoholo i ka ph o ka Bufer Kōkua.
• Nā pōkā phosphate pono i ka ph o ka pae o 6.0 a 8.0.
• Hiki iā ʻoe E hoʻomākaukau iā Phosphate Buiffers me ka hoʻohanaʻana Kh2po4 a K2hpo4, a iʻole ma ka heluʻana phosphorcus acid (H3po4) me kahi kumu ikaika.
• E makaʻala ph ʻO ka hoʻoponopono a me ka pilikia e pono ai ke kūleʻa no ka kūleʻa Bufer hoʻomākaukau.
• Ināʻoe e hoʻomākaukau nei i ka buffer mai H3po4 Tite me Koh a hiki i ka hopena E kiʻi i ka pH i makemakeʻia.
• E hele aku mai Kh2po4 i K2hpo4 pono e pono ai Hoʻohui Koh.
• No ka mea, e hoʻohana HCOL.

Hāʻawi kēia alakaʻi i kahi kumu kūpono no ka hoʻomaopopoʻana a me ka hoʻohanaʻana Nā pōkā phosphate I kāu hana. E hoʻomanaʻo mau e kūkākūkā mau i nā protocols kūikawā a me nā kulekele palekana no kāu mau hoʻokolohua. Pōmaikaʻi iā ʻoe.